Journal of Threatened Taxa | www.threatenedtaxa.org | 26 May 2017 | 9(5): 10187–10193

 

 

275477.jpg

 

 

A new species of sea squirt Rhopalaea bilobata (Ascidiacea: Diazonidae) from the Andaman Islands, India

Jhimli Mondal 1, C. Raghunathan 2 & Tamal Mondal 3

1,2,3 Zoological Survey of India, Andaman & Nicobar Regional Centre, National Coral Reef Research Institute, Haddo, Port Blair, Andaman & Nicobar Islands 744102, India

1 jmjhimli@gmail.com (corresponding author), 2 raghuksc@rediffmail.com, 3 t_genetics@yahoo.com

 

 

 

 

 

 

doi: http://doi.org/10.11609/jott.2620.9.5.10187-10193 | ZooBank: urn:lsid:zoobank.org:pub:93557703-066A-43F2-B763-9FD5A94C87E3

 

Editor: Rosana Moreira da Rocha, Universidade Federal do Paraná, Curitiba, Brasil. Date of publication: 26 May 2017 (online & print)

 

Manuscript details: Ms # 2620 | Received 23 April 2016 | Final received 06 April 2017 | Finally accepted 21 April 2017

 

Citation: Mondal, J., C. Raghunathan & T. Mondal (2017). A new species of sea squirt Rhopalaea bilobata (Ascidiacea: Diazonidae) from the Andaman Islands, India. Journal of Threatened Taxa 9(5): 10187–10193; http://doi.org/10.11609/jott.2620.9.5.10187-10193

 

Copyright: © Mondal et al. 2017. Creative Commons Attribution 4.0 International License. JoTT allows unrestricted use of this article in any medium, reproduction and distribution by providing adequate credit to the authors and the source of publication.

 

Funding: Ministry of Environment, Forest and Climate Change, Government of India.

 

Competing interests: The authors declare no competing interests.

 

Acknowledgements: Authors are grateful the Director, Zoological Survey of India, Kolkata for providing all round support to carry out studies. Thanks are also extended to the authorities of Department of Environment and Forests, Andaman and Nicobar Administration for providing logistic support during field surveys. Thanks are also extended to Dr. Rosana M. Rocha, Zoology Department, Universidade Federal do Paraná, Curitiba, PR, Brasil and Dr. Linda Cole, Smithsonian Institution, National Museum of Natural History, MRC-163, Dept. of Invertebrate Zoology for providing necessary literature for the confirmation of identification.

 

 

 

Abstracts: Rhopalaea bilobata, a new species of the class Ascidiacea, under the family Diazonidae has been described from the Andaman Islands of Andaman & Nicobar, India. The species was found in sandy bottoms, reef areas and artificial structures at a depth range of 10–40 m. This species has bilobed anal border, six lobed branchial and atrial siphons, transparent thorax with pigmented spots at the anus, between the two siphons and at the anterior end of endostyle, and ramified basal test. The species is closely related to R. idoneta, R. macrothorax and R. tenuis in several sets of anatomical and morphological features.

Keywords: Anal border, Andaman & Nicobar Islands, Ascidians, Rhopalaea, Tunicata.

 

 

 

 

Ascidians are invertebrates, sister group to vertebrates (Satoh 2009), that belong to the class Ascidiacea under the subphylum Tunicata. These sac-like sessile animals are exclusively marine in nature. About 3000 species of ascidians are found worldwide. The family Diazonidae is recently placed under the order Aplousobranchia (Shenkar et al. 2016) in which the genus Rhopalaea belongs. Presently, the genus has 20 accepted species across the world’s ocean (Shenkar et al. 2017). Most of the species belonging to this genus are reef associated though some can also be found on artificial reefs (Shenkar 2008, 2013). A total of 11 species under the genus are solitary and nine species are colonial but those do not form the large colonies like the species that belong to the genus Diazona (Shenkar 2013). Andaman & Nicobar Islands have extensive reef ecosystems in the continental shelf with a wide range of species abundance. A total of 65 species of ascidians are reported till date from the reefs of these islands (Venkataraman et al. 2012; Ananthan 2014; Ananthan et al. 2015; Mondal et al. 2015, 2017). The present paper describes one new species of Rhopalaea and gives its distribution in the Andaman Islands.

 

 

Materials and Methods

Specimens were collected by Self Contained Underwater Breathing Apparatus (SCUBA) diving. The surveys were conducted during October 2013 to December 2015 in various locations of the Andaman Islands (Fig. 1). Specimens were defecated with magnesium sulphate crystals (MgSO4.7H2O) and then narcotized with menthol crystals, following Meenakshi et al. (2003). After narcotization, specimens were preserved with 4% formaldehyde in seawater. Dissection was carried out under Labomed CZM4 microscope and digitization of detailed anatomy was carried out under Leica M205A DFC 500 stereo zoom microscope. Identification and comparison of anatomical as well as morphological features of the specimens followed by Kott (1990, 2006), Shenkar (2013), Monniot & Monniot (2001), Tokioka (1953, 1971), Monniot (1997), Sluiter (1904), Van Name (1921), Herdman (1880), Kott & Goodbody (2008), Vazquez & Young (1996), and Pérès (1958). The identified specimens were registered in National Zoological Collections and deposited at Zoological Survey of India, Port Blair.

316255.jpg

Results

Class: Ascidiacea Blainville, 1824

Order: Phlebobranchia Lahille, 1886

Family: Diazonidae Seeliger, 1906

Genus: Rhopalaea Philippi, 1843

 

 

Rhopalaea bilobata sp. nov.

(Images 1–5)

urn:lsid:zoobank.org:act:891BBC10-6664-4335-9613-9FB3548CF466

 

 

 

Material examined

Holotype: ZSI/ANRC-13703, collection of one specimen was made on 22.x.2013 from Wall area (12003.313’N & 92057.730E) located at Havelock Island, South Andaman at 30m depth (Fig. 1), Andaman & Nicobar Islands, India.

Syntypes: ZSI/ANRC-13704. Two specimens were collected on 22.x.2013 from the same location and depth of the holotype specimen and deposited in NZCs after detailed anatomical studies.

Paratypes: ZSI/ANRC-13705 & 13706. One specimen was collected on 16.xii.2013 from Trilby Island (13024.812’N & 93004.146’E) of North and Middle Andaman at 18m depth, while one specimen was collected from Pongibalu (11030.958’N & 92039.201’E) of South Andaman at 10m depth (Fig. 1).

 

 

Etymology

The species bears distinctive triangular bilobed anal border.

 

 

Description

External morphology: Zooids are usually solitary (Image 1ad), sometimes found in pairs (twice noticed underwater) but do not form large colonies. Zooids are finger like with soft translucent tunic and about 3.5cm (specimens not fully relaxed) - 5.6cm long. As in other species of Rhopalaea only the thorax is visible underwater and the abdominal part is concealed in the crevices or sand. Some specimens were collected from artificial substrate like tires and settlement plates.

The tunic delicate anteriorly (Image 2a) but became firm posteriorly (Image 2b), with round horizontal wrinkles on the surface. The tunic of the thoracic region free of epibionts. Basally the tunic produces some branched root-like extensions to adhere to the substrate, sometimes sand embedded in those branches and hard to remove (Image 2c,d). Both branchial and atrial apertures situated at the free anterior end, each with six lobes. Yellow dots present between siphons lobes. Specimens in live condition with a transparent test with a purple circular ring surrounding the branchial siphon and interrupted with ventral and dorsal yellow patches, covering the dorsal tubercle and the anterior end of the endostyle. Along with the endostyle, a yellow line found. Another yellow patch found surrounding the rectum under the anal border. Although, no colours persisted in the preservatives, traces found. A pinkish abdomen (colour faded in the preservatives) clearly seen through the test situated entirely posterior to the pharynx.

Internal structures: Large thorax gradually narrows posteriorly and a narrow oesophageal neck with a club shaped abdomen. One syntype specimen not fully relaxed, the thorax 0.5cm long and abdomen 0.8cm long. In relaxed specimens thorax 1.3–2 cm long and the abdomen 1–1.2 cm. The oesophageal neck very fragile and broke in most of the cases during dissection. Thorax with 10–14 longitudinal muscle bands on each side which arise from both siphons (Image 3a–c). Longitudinal muscles arising from the siphons, run half way of the pharynx and ramify towards the ventral and dorsal border: 6–7 longitudinal muscle bands arise from the branchial siphon and 3–5 from the atrial siphon and 1–2 muscle bands from the region in between the siphons. The branchial siphon with 9–11 circular muscles and the atrial siphon about six (Image 3d).

The dorsal tubercle aperture slit-like (slightly curved) in structure (Image 4a). Oral tentacles of two sizes and 18–22 long tentacles alternate with smaller ones. 28–32 internal longitudinal vessels present on each side and their number decreases (15–17) towards the oesophagus. Longitudinal vessels supported by papillae (Image 4b,c) and no record of secondary papillae. Three stigmata per mesh (Image 4d), in a relaxed specimen 54 rows of stigmata found (holotype) and 45 rows of stigmata in the syntype (not well relaxed). The dorsal lamina with 34–36 long triangular languets (Image 4c), starting at the third row of stigmata. The anterior portion of the colour patch along the endostyle wider than along the posterior part.

A vertical gut loop present embedded in the test. The gonads enclosed in the gut loop. Rectum long and opens at the 10th–13th row of stigmata (13th in not relaxed syntype) in the dorsal side. Anal border bilobed with two slightly indented triangular lips (40–65 µm) (Image 5a,b). The opening of oviduct just above the pigmented ring on the base of the anus and the sperm duct with slightly elongated papilla projected little above the oviduct but not beyond the anal border (Image 5c,d). Blood vessels of abdominal tunic continue in the ramifications of the root-like projections of the tunic (Image 2d).

 

316788.jpg

 

316789.jpg

 

316790.jpg

 

316791.jpg

Distribution

India: Havelock Island, Trilby Island and Pongibalu of Andaman Islands.

 

 

Discussion

A total of 20 species under the genus Rhopalaea were recorded across the world’s ocean till now while 11 of the species—R. abdominalis (Sluiter, 1898), R. birkelandi Tokioka, 1971, R. cloneyi Monniot & Monniot, 2001, R. crassa (Herdman, 1880), R. flemingi (Herdman, 1880), R. idoneta Shenkar, 2013, R. macrothorax Tokioka, 1953, R. meridionalis Kott, 2006, R. neapolitana Philippi, 1843, R. perlucida Monniot, 1997 and R. tenuis (Sluiter, 1904)—are solitary, and nine species—R. circula Monniot & Monniot, 2001, R. defecta (Sluiter, 1904), R. desme Monniot, F. 2003, R. fusca (Herdman, 1880), R. hartmeyeri (Salfi, 1927), R. orientalis Pérès, 1958, R. piru Monniot & Monniot, 1987, R. respiciens Monniot, 1991, R. sagamiana Oka, 1927—are colonial (Shenkar, 2013). Rhopalaea bilobata sp. nov. has a remarkable resemblance to R. idoneta and R. macrothorax. The similarity with R. idoneta includes the transparent thorax, branching structures for fixation and similar anus shape. The differences include a colourless thorax, the muscular pattern, 4–5 stigmata per mesh, the position of the anus and gonoduct posterior to the branchial sac with the two lobes of the anus deeply divided into lobes, and eight lobed atrial aperture in R. idoneta (Shenkar 2013).

The external morphology of the presently described species is quite similar to R. macrothorax, also present in the Andaman Islands (Image 6a,b), except for the purple endostyle in R. macrothorax which is yellow in R. bilobata sp. nov. R. macrothorax in our region has 26 oral tentacles, in the range described by other researchers (Tokioka 1953; Monniot & Monniot 2008). Musculature pattern is quite similar (Image 6c,d) as shown by Monniot & Monniot (2008). But R. macrothorax has more longitudinal vessels (42) and rows of stigmata (62) than R. bilobata sp. nov. The most prominent difference is the anal border and gonoduct openings (Image 6e,f); R. macrothorax has a smooth and thick anal border and not triangular; the sperm duct is longer and opens beyond the anal border.

The other two reported species in the region are R. circula and R. fusca which are colonial and are restricted to the Nicobar group of islands. Apart from being colonial, R. circula also has more muscles, rows of stigmata, longitudinal vessels than R. bilobata sp. nov. and a different anal and gonadal opening. The pattern of the longitudinal muscles of the new species is similar to R. fusca (Monniot & Monniot 2001), but R. bilobata sp. nov. has fewer longitudinal vessels and rows of stigmata in comparison with R. fusca and it is transparent.

Distinctive differences can be seen with R. perlucida which has eight lobed branchial siphon, longitudinal muscles on thorax not ramified towards the dorsal and ventral borders, only eight rows of stigmata along with 50–70 longitudinal vessels and anus located posterior of the third row Monniot 1997). R. tenuis has the same basal root-like projections which are also found in the described species, but has fewer longitudinal vessels (12), 20 longitudinal muscles of which half are originated from the branchial siphon and other half originated from the atrial siphon and four stigmata per mesh (Kott 1990).

 

 

References

 

 

Ananthan, G. (2014). Diversity and Ecology of Sedentary Ascidians of the Great Nicobar Biosphere Reserve. First Year Progess Report, 103pp.

Ananthan, G., C. Stalin & C. Raghunathan (2015). A new record of ascidians in Indian waters, from the Great Nicobar Biosphere Reserve, Andaman and Nicobar Islands. Marine Biodiversity Records 8: 1–5.

Herdman, W.A. (1880). Preliminary report on the TUNICATA of the “Challenger” Expedition. The Royal Rociety of Edinburgh Proceedings 10: 458–472.

Kott, P. & I. Goodbody (1980). The Ascidians of Hong Kong, pp. 503–554. In: Morton, B. & C.K. Tseng (eds.). Proceedings of the First International Marine Biological Workshop: The Marine Flora and Fauna of Hong Kong and Southern Chaina. Vol. 1. Introduction and Taxonomy. Hong Kong University Press.

Kott, P. (1990). The Australian Ascidiacea. Part 2. Aplousobranchia (1). Memoirs of Queensland Museum 29(1): 1–298.

Kott, P. (2006). Observations on non-didemnid ascidians from Australian waters (1). Journal of Natural History 40(3): 169–234; http://doi.org/10.1080/00222930600621601

Meenakshi, V.K., T.K. Renganathan, S. Sentharamarai & J. Jeyalakshmi (2003). Marine Biodiversity - Taxonomy of Indian Ascidians. Technical Report, 38pp.

Mondal, J., C. Raghunathan & T. Mondal (2015). Diversity and Distribution of common ascidians of Andaman group of Islands. Middle East Journal of Scientific Research 23(10): 2411–2417.

Mondal, J., C. Raghunathan, T. Mondal & K. Chandra (2017). Reef Associated ascidians of Andaman and Nicobar Islands. Records of Zoological Survey of India, Occasional No. 384: 1-108.

Monniot, C. (1997). Ascidies phlebobranches du canal du Mozambique. Zoosystema 19(4): 557–571.

Monniot, F. & C. Monniot (2001). Ascidians from the tropical western Pacific. Zoosystema 23: 201–383.

Monniot, F. & C. Monniot (2008). Compléments sur la diversité des ascidies (Ascidiacea, Tunicata) de l’ouest Pacifique tropical. Zoosystema 30(4): 799–872.

Pérès, J.M. (1958). Ascidies de la Baie de Haifa Collectées par E. Gottlieb. Bulletin of the Research Council Of Israel 78: 151–164.

Satoh, N. (2009). An advanced filter-feeder hypothesis for urochordate evolution. Zoological Science 26(2): 97–111.

Shenkar, N. (2008). Ecological aspects of the ascidian community along the Israeli coasts. PhD Thesis. Tel-Aviv University, Israel, 122pp.

Shenkar, N. (2013). A new species of the genus Rhopalaea (Class: Ascidiacea) from the Red Sea. Zootaxa 3599(1): 051–058.

Shenkar, N., G. Koplovitz, L. Dray, C. Gissi & D. Huchon (2016). Back to solitude: Solving the phylogenetic position of the Diazonidae using molecular and developmental characters. Molecular Phylogenetics and Evolution 100: 51–56.

Shenkar, N., A. Gittenberger, G. Lambert, M. Rius, R.M. Rocha, B.J. Swalla & X. Turon (2017). Ascidiacea World Database. Accessed at http://www.marinespecies.org/ascidiacea on 2016-12-14

Sluiter, C.P. (1904). Die Tunicaten der Siboga-Expedition. Part-I: Die socialen und holosomen Ascidien. Siboga Expedition 56(A): 1–139.

Tokioka, T. (1953). Contribution to Japanese ascidian fauna. 5. Ascidians collected near the Marine Biological Laboratory of Hiroshima University in the Inland Sea. Publications of the Seto marine biological Laboratory 3(1): 1–25.

Tokioka, T. (1971). A new species of Rhopalaea from the Pacific Coast of Costa Rica (Tunicata, Ascidiacea). Publications of the Seto marine biological Laboratory XIX(2/3): 119–122.

van Name, W.G. (1921). Ascidians of the West Indian Region and Southern United States. Bulletin American Museum of Natural History XLIV: 283–494.

Vazquez, E. & C.M. Young (1996). Rhopalaea cloneyi (Ascidiacea, Cionidae), a new deep-water ascidian from the coastal waters of British Columbia. Journal of Zoology, London 238: 599–610.

Venkataraman, K., C. Raghunathan, R. Raghuraman, & C.R. Sreeraj (2012). Marine Biodiversity in India. Zoological Survey of India, Kolkata, 168pp.

Short Communication