Note

150574.png

Taxonomic notes on stingless bee Trigona (Tetragonula) iridipennis Smith (Hymenoptera: Apidae) from India

 

K. Vijayakumar 1 & R. Jeyaraaj 2

 

1,2 Department of Zoology, Kongu Nadu Arts and Science College, G.N. Mills, Coimbatore, Tamil Nadu 641029, India

1 knvijgs@gmail.com (corresponding author), 2 jeyaraaj_indira@yahoo.co.in

 

 

doi: http://dx.doi.org/10.11609/JoTT.o3773.6480-4 | ZooBank: urn:lsid:zoobank.org:pub:0DADA717-8726-4D19-A5CA-9E858669C072

 

Editor: P.M. Sureshan, Zoological Survey of India, Calicut, India. Date of publication: 26 October 2014 (online & print)

 

Manuscript details: Ms # o3773 | Received 16 September 2013 | Final received 09 August 2014 | Finally accepted 29 September 2014

 

Citation: Vijayakumar, K. & R. Jeyaraaj (2014). Taxonomic notes on Trigona (Tetragonula) iridipennis Smith (Hymenoptera: Apidae) from India. Journal of Threatened Taxa 6(11): 6480–6484; http://dx.doi.org/10.11609/JoTT.o3773.6480-4

 

Copyright: © Vijayakumar & Jeyaraaj 2014. Creative Commons Attribution 4.0 International License. JoTT allows unrestricted use of this article in any medium, reproduction and distribution by providing adequate credit to the authors and the source of publication.

 

Funding: None.

 

Competing Interest: The authors declare no competing interests.

 

Acknowledgements: We are grateful to Dr. Deborah Smith from University of Kansas, USA for helping in identifying the stingless bee species and useful comments. I also express my sincere thanks to Dr. M. Muthuraman, for his encouragement and motivation to pursue the research work. I would also like to thank beekeepers Mr. K. Udayakumar, Mr. G.K. Thangavelu, Mr. Navaneethan from Nellithurai Village for their participation during sample collection and documentation.

 

189819.jpg

 

Stingless bees are a monophyletic group principally found in the tropical and subtropical areas of America, Africa, Australia and parts of Asia (Roubik 1989). The stingless bee species are taxonomically organized into two major genera viz., Trigona, the largest group and Melipona, a genera consisting exclusively of the genus Melipona. Most of the Asian and African stingless bee species belong to the genera Trigona (Michener 2013). Taxonomic identification of stingless bees remains unclear and requires experienced taxonomists. The total number of species is estimated to be about 500 described species worldwide (Michener 2013) and 43 species occur in the Asian region (Michener 2000).

The scientific genus-group names for the different stingless bees from India have long been Trigona and Lisotrigona. Tetragonula is the single largest and most widespread genus in the Indo-Malayan regions, reported from India and extending to the Solomon and Caroline Islands. Trigona iridipennis is the type species of the subgenus Tetragonula (Sakagami 1978; Michener 2013; Rasmussen 2013) and this species was originally described from Ceylon by Smith in the year 1854.

Studies of species diversity of Trigona in different parts of India are lacking. Scarce literature is available on stingless bee species diversity in India. The distribution of T. iridipennis was reported in Bengaluru, Karnataka (Biesmeijer 1993) and in Kerala (Mohan & Devanesan 1999).

The diversity of stingless bees in the Indian subcontinent has been summarized by Rasmussen (2013). The “iridipennis” species group is extremely similar in external morphology of the workers and the morphological characteristics of the male genitalia and molecular data of the species are needed to separate the species from the “iridipennis” group. Vijayakumar & Jayaraj (2013) discriminate the three species (T. iridipennis, T. laeviceps and Lepidotrigona arcifera) of stingless bees by using relative warp analysis of the forewings from India. The present study reports the morphological key characteristics of male and female bees of Tetragonula iridipennis in Nellithurai Village, Tamil Nadu, India.

Most of the researchers in India concentrated only on stingless bee biology, morphometry, natural enemies and its pollination biology (Muthuraman & Saravanan 2004; Danareddi & Viraktamath 2009; Vijayakumar et al. 2012). The studies on stingless bee species diversity in India are rare and our understanding of their identification is at an early stage.

 

Materials and Methods

Sample collection: The adult worker samples from nest entrance and male bees during the swarming period were collected at Nellithurai (11016’59.74”N & 76053’6.24”E), Tamil Nadu, India (Image 1) and preserved in 90% ethyl alcohol for further identification. Taxonomic identification was based on the nest architecture and morphology according to Sakagami et al. (1983) and Schwarz (1939). The preserved male and female stingless bees were dissected and the morphological characteristics of the worker and drone stingless bees were photographed by using a Leica M165C stereo microscope with image analyzer. The male genitalia were cleared in a 0.5% KOH solution and examined in both wet and dry conditions. In addition, male sternum V and sternum VI were used as a key characteristic for distinguishing the Tetragonula stingless bees used by Schwarz (1939). Species identity was confirmed by Dr. Deborah Smith, Department of Ecology and Evolutionary, Biology/Entomology, the University of Kansas, USA.

 

189818.jpg

 

Results

The identity of the Trigona (Tetragonula) iridipennis Smith, depends strongly on the works of Schwarz (1939), Sakagami (1978) and Sakagami & Inoue (1987) for the subgeneric characterization and for species recognition. These authors indicated that the size differences as well as male genitalia and sternal characters are important key morphological characteristics for identifying the species. The species descriptions are given below.

Description: T. iridipennis is the type species of the subgenus Tetragonula. This species has formerly been regarded as very widespread from India to Solomon Island. The “iridipennis” species group is the largest and most widespread group in the Indo-pacific areas.

Diagnostic characters of worker (Images 2 a–h): The malar space is vestigial and the scutellum is extended backward. The mandible with two well developed teeth on the inner half of its apex. The basal sericeous area positioned more than half of the length of basitarsus.

Metric values: The total body length ranges from 3.5–4.0 mm, and head width ranges from 1.50–1.68 mm; forewing length includes tegula ranging from 3.2–3.9 mm, bifurcation between vein m and cu ranges from 0.90–1.12 mm and hind tibial length ranges from 1.29–1.57 mm.

Colour and pilosity: The entire body is black to blackish-brown. The clypeus, tegula, legs and metasoma are dark in colour (Image 2a). The frontal hairs are fulvous to whitish and plumose (Image 2b). The mesoscutal hairs well banded and fulvous to testaceous in colour (Image 2c). The mesoscutellar fringes are fulvous to testaceous. The erect hairs of the mesopleura are silvery to white in colour. The frontal hairs are mostly fulvous to whitish (Image 2d). The anterior veins and stigma of forewings are dark brown.

The hairs fringing on the hind tibiae posterior are plumose and the outer surface of hind tibia with dark brown stout setae (Image 2e). The hairs on upper surface of thorax is mostly light in colour and the hairs fringing the anterior contour of the hind tibiae are black (Image 2f). The basal and apical half of the wings are uniform in colour and the anterior veins and stigma dark brown (Image 2g). The number of hamuli on hind wing is constantly five per wing (Image 2h).

 

 

189817.jpg

 

 

 

Diagnostic characters of male (Image 3 a–h): The structural characteristics are more similar to female bees (Images 3a–c). The posterior margin of basitarsus is imperceptibly angulate. The outer surface of the hind tibiae medially gently convex and apically slightly depressed (Image 3d).

Metric values: The total body length ranges from 2.5–3.5 mm, head width ranges from 1.38–1.43 mm, forewing length including tegula ranges from 3.1–3.8 mm, bifurcation between m and cu ranges from 0.88–0.95 mm; hind tibial length ranges from 0.96–1.23 mm.

Colour and pilosity: The male stingless bees are similar to female bees in colour characteristics. The dense plumose hairs cover the posterior fringe of the mid tibia. The hairs on the outer surface of the hind tibiae are plumose and sparser (Image 3e,f). The sternum V (S5) is small and the sternite VI (S6) is antegladular area medially long and postgladular area short (Image 3g). The median depression of sternite VI with sparse hairs and the apex is narrowly and shallowly incised.

Genitalia: The male genitalia are one of the most important characteristics for taxonomic study. The gonostylus is long and slender, more or less sinuous with sparse hairs at apex, penis valve is very robust, tapering only at the apex, about as long as or slightly shorter than gonostylus. The gonostylus arises from the dorsum of gonocoxite (Image 3h).

 

189816.jpg

Discussion

Stingless bees are a tropical group of over 500 species worldwide (Michener 2013). Recently, Rathor et al. (2013) referred to seven stingless bee species in India. Rasmussen (2013) listed eight species from the Indian subcontinent and T. iridipennis is a wide spread species in India. He pointed out that the species of the iridipennis group are extremely similar in external morphology of the workers and a taxonomic revision of the species of India should include morphological characteristics of the male genitalia. The present study reports the key morphological characteristics of worker bees and male genitalia.

The iridipennis group is characterized by having a dark mesoscutum with four distinct hair bands separated by broad glabrous interspaces. The genital morphology and molecular data are needed for correctly describing the iridipennis species group. Because the iridipennis species group is extremely similar in external morphology to the workers, most of the authors pointed out that the mesoscutum with glabrous interspaces are the key characteristics for the iridipennis species group (Schwarz 1939; Sakagami 1978; Dollin et al. 1997; Rasmussen 2013). Based on the structure of male genitalia, Sakagami (1978) includes four species (T. iridipennis, T. clypearis, T. fuscobalteata and T. pagdeni) under the iridipennis group.

The present study reports the existence of Tetragonula iridipennis from the iridipennis group in Nellithurai village, Tamil Nadu, India. The female bees were morphologically similar and are marked with minor differences in hairs on head and thorax, sereceous space and marginal cell in forewing. The male genitalia are the best diagnostic characteristics for differentiating within iridipennis group, particularly, the shapes of the various parts of genitalia. The gonostylus of T. iridipennis is long and slender and sinuous with sparse hairs at apex. The penis valve is very robust and tapering only at the apex.

 

References

Biesmeijer, K. (1993). Stingless bees: discussion and paper at the International symposium on pollination tropics Pegone, 1: 6–8.

Danareddi, C.S. & S. Viraktamath (2009). Morphometrical studies on the stingless bee, Trigona iridipennis Smith Karnataka Journal of Agricultural Sciences 22: 796–797.

Michener, C.D. (2000). The Bees of The World. Johns Hopkins University Press, Baltimore, xiv+[1]+913.

Michener, C.D. (2013). The Meliponini, pp. 3–17. In: Vit, P., S.R.M. Pedro & D.W. Roubik (eds.). Pot-Honey: A Legacy of Stingless Bees. Springer, New York.

Mohan, R. & S. Devanesan (1999). Dammer Bees, Trigona iridipennis Smith. (Apidae: Meliponinae) in Kerala. Insect Environment 5(2): 79.

Muthuraman, M. & P.A. Saravanan (2004). Utilization of Stingless Bees for crop pollination. Indian Bee Journal 66: 58–64.

Rasmussen, C.A. (2013). Stingless Bees (Hymenoptera: Apidae: Meliponini) of the Indian subcontinent: Diversity, taxonomy and current status of knowledge. Zootaxa 3647(3): 401–428; http://dx.doi.org/10.11646/zootaxa.3647.3.1

Rathor, V.S., C. Rasmussen & M.S. Saini (2013). New record of the Stingless Bee Tetragonula gressitti from India (Hymenoptera: Apidae: Meliponini). Journal of Melittology 7: 1–5.

Roubik, D.W. (1989). Ecology and Natural History of Tropical Bees. Cambridge University Press, New York, 514pp.

Sakagami, S.F. (1978). Tetragonula Stingless Bees of the continental Asia and Sri Lanka (Hymenoptera: Apidae). Journal of the Faculty of Science, Hokkaido University, Series VI, Zoology 21: 165–247.

Sakagami, S.F., S. Yamane & G.G. Hambali (1983). Nests of some southeast Asian stingless bees. Bulletin of the Faculty of Education, Ibaraki University (Natural Sciences) 32: 1–21.

Sakagami, S.F. & T. Inoue (1987). Stingless Bees of the genus Trigona (subgenus Trigonella) with notes on the reduction of spatha in male genitalia of the subgenus Tetragonula (Hymenoptera, Apidae). Kontyû 55: 610–627.

Schwarz, H.F. (1939). The Indo-Malayan species of Trigona. Bulletin of the American Museum of Natural History 76: 83–141.

Vijayakumar, K. & K.R. Jayaraj (2013). Geometric morphometry analysis of three species of stingless bees in India. International Journal for Life Sciences and Educational Research 1(2): 91–95.

Vijayakumar, K., M. Muthuraman & R. Jayaraj (2012). Predation of stingless bees (Trigona iridipennis: Apidae, Meliponinae) by centipede (Scolopendra hardwicki: Chilopoda: Scolopendramorpha). International Journal of Advanced Life Sciences 5(2): 156–159.